Hana:
I work with picomole amounts of material often in my DNA repair studies. When
in doubt, label the DNA with 32P, resolve by PAGE, visulize with autoradiogram,
and elute by electroelution into a centricon. The protocols are in my
publications. It is easy and the yield is high. What you see is what you
get.
Tony
At 12:41 PM 1/22/2001 +0100, =?iso-8859-2?Q?RNDr._Hana_Kone=E8n=E1?= wrote:
>
> Dear colleagues,
>
> I have a researcher asking for a separation of tiny amounts of DNA oligomers
> (25-mer, 50-mer and 55-mer) in one fraction from the 80bp hybridization
> product. The hybridization product has to be clean for further ligation
> experiments. Because of very low concentration of the product I am hesitating
> to use a gel filtration. Does anyone have any experience using
> ultrafiltration spin-columns (such as the Amicon or the Eppendorf sell) for
> nucleic acids. I am especially concerned about non-specific membrane sorption
> (like some proteins do...). Thanks for your input.
>
> Hana
>
>
> Hana Konecna
> Core Facility
> Laboratory of Functional Genomics and Proteomics
> Faculty of Science, Masaryk University
> Kotlarska 2, 611 37 Brno, Czech Republic
>
> <mailto:hanak@sci.muni.cz>hanak@sci.muni.cz
> http://www.sci.muni.cz/LMFR/
> Phone 420-5-411 29 369
> FAX 420-5-411 29 372
>
>
>
>
>
************************************
Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave., Philadelphia, PA 19111
Voice: 215-728-2488 FAX: 215-728-3647 email: AT_Yeung@FCCC.edu
Public Key: D994 4658 6AE2 12A6 A538 D18C 461F 5ED2 21C5 2AFF
http://www.fccc.edu/research/labs/yeung/
************************************
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