Anyone having experience with both activators:
Please respond per your experience in terms of completeness of coupling. I
understand that HATU is "faster" in coupling than HBTU. But...is it better in
that you get a higher percentage of sites coupled with the amino acid in
question.
I have experienced incomplete coupling of the first amino acid onto
Fmoc-L-Lys-(e-BiotinLC)0OH to Rink amide MBHA resin. This is the first time I
have used this resin and I am told the coupling may be difficult. So, I'm
thinking I should couple the first amino acid onto the resin overnite. And I'm
wondering if I would be better off using HATU instead of HBTU?
Thanks in advance,
Millie McAdams
HHMI Biopolymer Facility @ DUMC
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