Amanda,
one reference would be
van Oostveen I, Ducret A, Aebersold R (1997) Colloidal silver staining of electroblotted proteins for high sensitivity peptide mapping by liquid
chromatography-electrospray ionization tandem mass spectrometry Anal Biochem 1997 May 1;247(2):310-8.
Axel
Amanda Hall wrote:
> Hi Guys,
>
> A client has sent me sample for internal digestion. It is on nitrocellulose. What is the best way to go about it (blocking solution, digestion solution)
>
> Thanks alot
>
> Amanda
>
> |\ _,,,---,,_
> zz /,`.-'`' -. ;-;;,_
> |,4- ) )-,_..;\ ( `'-'
> '---''(_/--' `-'\_)
>
> Amanda Hall-Griffin
> Professional Officer
> Newcastle Protein
> University of Newcastle
> Ph (02) 4921-7299
> Fax (02) 4921-6903
-- ------------------------------------------------------------------------Axel Ducret, Ph.D. Senior Research Biologist Merck-Frosst Canada Inc. Dept. Biochemistry and Molecular Biology P.O. Box 1005 Pointe-Claire-Dorval PQ H9R 4P8 Canada
tel. + (514) 428-3428 fax + (514) 428-4900
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