Alan- We use the membrane combs (from Gel Co.) for sequencing reactions on both the 373 and 377 without prerunning at all. We think they are great.
Allison Pinder DNA Sequencing Core Facility
HHMI/Carnegie Institution of Washington
Department of Embryology
115 West University Parkway
Baltimore, MD 21210
(410) 554-1207
pinder@ciwemb.edu
---------- Original Message ----------------------------------
From: "Helix Biotech" <helix@execpc.com>
Date: Wed, 31 Jan 2001 15:26:03 -0600
>I would like to use Gel Company membrane combs for fragment analysis on an
>ABI 373. Normally, I mount the upper buffer reservoir and pre-run the gel to
>40oC. However, with the membrane combs, they are inserted prior to mounting
>the buffer reservoir with no pre-run. Anyone have experience with membrane
>combs on the 373? Is pre-running necessary?
>
>Thanks... Alan
> Helix Biotech, Inc.
>
>
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