Dear ABRFer,
This is Takeshi from Yokohama in japan.
I plan to do on-stage ladder sequencing.
Carboxypeptidase and Amnopeptidase are there.
Protocol is shown in Practical Approach
[Protein Phosphorylation 2nd edition.]
MALDI-TOF is Voyger DE RP.
Question is.....
Which peptidase is well working in your lab?
C-peptidase Y is provided from
Pierce
Boehringer
C-peptidase Y is provided from
Boehringer
N-peptidase M is provided from
Sigma
If you have experiences to use enzyme showb above,
please advice me the selection of best vendor.
I also want to know detail techniques and remark out
of practical approach.
Thanks in advance.
Takeshi Fukuhara
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Takeshi FUKUHARA
Laboratory of Cell and Developmental Biology (c/o Dr.Takeo Kishimoto),
Department of Biological Sciences,
Graduate School of Biosciences and Biotechnology,
Tokyo Institute of Technology.
B2-833 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan
Phone : 81-45-924-5725/Fax : 81-45-924-5738
e-mail: tfukuhar@bio.titech.ac.jp
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