Hello~:
I am trying phtoaffinity labeling with using biotinylated photo-activatable
crosslinker. Recently I found that many protein from tissue (CNS) can bind
with stretavidin-HRP even without being labeled with cross-linker. So I want
to use anti-biotin antibody instead of avidin/streptavidin to detect
biotinylated protein in western blotting. Could you recommend the
commercially available good anti-biotin antibody (in terms of specificity)?
I found some of them in catalogues of Pierce and Jackson Lab.
Many thanks,
Young-Joon KIM
UC Riverside
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