methionine oxidation by H2O2

From: VERNON SHOUP (vernon.shoup@regpha.com)
Date: Wed Feb 07 2001 - 10:49:42 EST

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Hello.

I want to study the kinetics of methionine oxidation by hydrogen peroxide (Met => MetO) in one of our proteins . My problem is that I don't know how to stop the reaction after various time points. I'm thinking about adding an excess of a reducing agent, like sodium dithionite or DTT. Of course, I want to stop the reaction in a way that doesn't further damage the protein or reduce any methionine oxide.
Does anyone have any comments or suggestions about this?

Thank you!

Vernon

Vernon A. Shoup
Regeneron Pharmaceuticals
Rensselaer, NY 12144

vernon.shoup@regpha.com

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