Sergey, You could get by with a semi-prep flow cell just by monitoring at
280 nm rather than 220--and then moving off of the top of that peak, like
say to 260 which is close to phenylalanines 258 absorbance, if you
need to get the signal down to an even lower level.
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403
On Wed, 7 Feb 2001, Sergey V. Burov wrote:
> Dear All,
>
> We have bought System Gold from Beckman for the preparative purification
> of peptides. However, due to the supplier mistake
> it includes analytical flow cell and isnít valuable for the preparative
> runs. So, we should change the flow cell. The question is: What type of
> Beckman cell (prep or semi-prep) is satisfy our aims?
> Typical purification conditions are:
> Sample concentration - 50 mg/ml (or more)
> Sample volume - 2 ml
> Flow rate 10 ml/min (or more)
> Our domestic Beckman affiliate canít give us an advice in this regard.
> Preliminary Internet search was also unsuccessful.
>
> With best regards,
> Sergey Burov
>
> Institute of Macromolecular Compounds,
> 199004, V.O., Bolshoi pr. 31,
> St.-Petersburg, Russia
>
> Tel: 7 (812) 323 36 07
> Fax: 7 (812) 328 68 69
> Email: burov@hq.macro.ru
> diapharm@diapharm.spb.ru
>
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