Sergey,
10 ml/min will not break your analytical cell. To avoid overscaling,
just shift the wavelength to lower extinction (e.g. to 230 nm from
220)... and save your money. Generally, 10 ml/min correspond to
semi-prep cell.
Vladimir
On 07/02/2001, Sergey V. Burov wrote:
> Dear All,
> We have bought System Gold from Beckman for the preparative purification
> of peptides. However, due to the supplier mistake
> it includes analytical flow cell and isnít valuable for the preparative
> runs. So, we should change the flow cell. The question is: What type of
> Beckman cell (prep or semi-prep) is satisfy our aims?
> Typical purification conditions are:
> Sample concentration - 50 mg/ml (or more)
> Sample volume - 2 ml
> Flow rate 10 ml/min (or more)
> Our domestic Beckman affiliate canít give us an advice in this regard.
> Preliminary Internet search was also unsuccessful.
> With best regards,
> Sergey Burov
> Institute of Macromolecular Compounds,
> 199004, V.O., Bolshoi pr. 31,
> St.-Petersburg, Russia
> Tel: 7 (812) 323 36 07
> Fax: 7 (812) 328 68 69
> Email: burov@hq.macro.ru
> diapharm@diapharm.spb.ru
Vladimir Titov
Bokiron Ltd., Moscow, Russia
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