Re: Prep or semi-prep?

From: MSweeney (mattsweeney@earthlink.net)
Date: Wed Feb 07 2001 - 09:29:11 EST


Sergey
I don't know system gold details but...
the gilson i am using has a 12mm long
cell on the analytical and a 0.2mm long
cell on the prep. The stuff we do is
around your conc. levels. I don't have many
hours on it myself yet so take it with
a grain of salt.

Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software

-----Original Message-----
From: Sergey V. Burov <burov@hq.macro.ru>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Wednesday, February 07, 2001 3:36 AM
Subject: Prep or semi-prep?

> Dear All,
>
>We have bought System Gold from Beckman for the preparative purification
>of peptides. However, due to the supplier mistake
>it includes analytical flow cell and isn't valuable for the preparative
>runs. So, we should change the flow cell. The question is: What type of
>Beckman cell (prep or semi-prep) is satisfy our aims?
>Typical purification conditions are:
>Sample concentration - 50 mg/ml (or more)
>Sample volume - 2 ml
>Flow rate 10 ml/min (or more)
>Our domestic Beckman affiliate can't give us an advice in this regard.
>Preliminary Internet search was also unsuccessful.
>
> With best regards,
> Sergey Burov
>
>Institute of Macromolecular Compounds,
>199004, V.O., Bolshoi pr. 31,
>St.-Petersburg, Russia
>
>Tel: 7 (812) 323 36 07
>Fax: 7 (812) 328 68 69
>Email: burov@hq.macro.ru
>diapharm@diapharm.spb.ru
>
>



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