I have a very inexpensive ISCO system,which started as an analytical
system.They have what they call a prep pump head,which can take up to
28ml/min.The pump head,the check valves,and all the plumbing have to be
increased in size,but the flow cell remained the same,with no ill effect.
I typically do not like to run more than 50mg at a time.This way I do not
have to worry about losing everything ,if something goes wrong.My flow
rate,with a 2.1cm column has been 8-10 ml/min.My separations are much
better this way.
I have been using semi-prep columns,1.0cm in diameter on the same
system,at 2-2.5ml/min with good results.
"Sergey V. Burov" <burov@hq.macro.ru>@aecom.yu.edu> on 02/07/2001 03:17:40
AM
Sent by: Association of Biomolecular Resource Facilities
<abrf-request@aecom.yu.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc:
Subject: Prep or semi-prep?
Dear All,
We have bought System Gold from Beckman for the preparative purification
of peptides. However, due to the supplier mistake
it includes analytical flow cell and isnít valuable for the preparative
runs. So, we should change the flow cell. The question is: What type of
Beckman cell (prep or semi-prep) is satisfy our aims?
Typical purification conditions are:
Sample concentration - 50 mg/ml (or more)
Sample volume - 2 ml
Flow rate 10 ml/min (or more)
Our domestic Beckman affiliate canít give us an advice in this regard.
Preliminary Internet search was also unsuccessful.
With best regards,
Sergey Burov
Institute of Macromolecular Compounds,
199004, V.O., Bolshoi pr. 31,
St.-Petersburg, Russia
Tel: 7 (812) 323 36 07
Fax: 7 (812) 328 68 69
Email: burov@hq.macro.ru
diapharm@diapharm.spb.ru
This archive was generated by hypermail 2b29 : Fri Feb 23 2001 - 13:03:34 EST