when we did a similar study we stopped the reaction with an excess of methionine. Worked very well.
Sheila G. Magil
Waratah Pharmaceuticals Inc.
VERNON SHOUP wrote:
> Hello.
>
> I want to study the kinetics of methionine oxidation by hydrogen peroxide (Met => MetO) in one of our proteins . My problem is that I don't know how to stop the reaction after various time points. I'm thinking about adding an excess of a reducing agent, like sodium dithionite or DTT. Of course, I want to stop the reaction in a way that doesn't further damage the protein or reduce any methionine oxide.
> Does anyone have any comments or suggestions about this?
>
> Thank you!
>
> Vernon
>
> Vernon A. Shoup
> Regeneron Pharmaceuticals
> Rensselaer, NY 12144
>
> vernon.shoup@regpha.com
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