Enrico,
Welcome to the wonderful world of PepSyn! I ask all my new entry level
employees to read the excellent (if slightly dated) review by Fields and
Noble in IJPPR as a starting point. It's probably more than you'll need for
a single synthesis of a dipeptide, but you'll appreciate the references.
Gregg B Fileds and Richard L Noble (1990) "Solid phase peptide synthesis
utilizing 9-fluorenylmethoxycarbonyl amino acids," Int. J. Peptide Protein
Res., Vol. 35, pp. 161-214.
There are also a couple of good books around. They may be harder for you to
find if you are using a decent library, but easier if you have to buy your
own literature. One of the more recent comprehensive books is:
Methods in Molecular Biology, Vol 35 Peptide Synthesis Protocols (1994) ed.
M W Pennington and B M Dunn, Humana Press, Totowa, New Jersey.
and its companion, Vol 36 Peptide Analysis Protocols, ed. B M Dunn and M W
Pennington.
Mark
-----Original Message-----
From: Enrico Millo [mailto:millo@vega.cba.unige.it]
Sent: Wednesday, February 07, 2001 11:41 AM
To: Recipients of ABRF List
Subject: information request
Dear ABRF,
> my research field is peptide synthesis. I have to make a dipeptide
>formed by Tyr-Phe in solution phase, can you give me some
>information about a protocol to use or some reference about this
>synthesis(reagent, conditions of coupling, best solvent)?
>Thank a lot
>Enrico Millo
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