Help with TBP

From: Michael N. Horst, Ph.D. (horst_mn@Mercer.EDU)
Date: Fri Feb 09 2001 - 10:49:58 EST


Hello abrfers-
    Over the last few months, I have been considering the use of
tributyl phosphine (TBP) after reading some encouraging posts to this
group, regarding its use as a reducing agent in the IEF dimension of 2-D
PAGE. However, when the reagent arrived here, I learned it is extremely
air-sensitive and prone to explosion in air. I would like to ask if any
of you who use this reagent on a regular basis could inform us about
your routine methods for handling this reagent, diluting it and storing
the now opened bottle of concentrated reagent. I thank you for your
time.
Cheahs,
Mike





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