We use a Hamilton syringe to remove just what we need from the bottle. The
bottle that we have has a rubber septum top and therefore stays sealed (or
at least relatively so). We immediately aliquot out the amount needed into
our solubilization buffer. The stock bottle is stored in the hood at room
temp. For us, we buy the smallest bottle possible and therefore we get
fresh TBP every few months.
Jim Lawrence
Pioneer Hi-Bred
-----Original Message-----
From: Michael N. Horst, Ph.D. [mailto:horst_mn@Mercer.EDU]
Sent: Friday, February 09, 2001 9:50 AM
To: Recipients of ABRF List
Subject: Help with TBP
Hello abrfers-
Over the last few months, I have been considering the use of
tributyl phosphine (TBP) after reading some encouraging posts to this
group, regarding its use as a reducing agent in the IEF dimension of 2-D
PAGE. However, when the reagent arrived here, I learned it is extremely
air-sensitive and prone to explosion in air. I would like to ask if any
of you who use this reagent on a regular basis could inform us about
your routine methods for handling this reagent, diluting it and storing
the now opened bottle of concentrated reagent. I thank you for your
time.
Cheahs,
Mike
This archive was generated by hypermail 2b29 : Fri Feb 23 2001 - 13:03:34 EST