Question, anyone have experience with determining aggregate formation in
protein solutions? I am in need of a good method/instrument to quantitate
both soluble and insoluble aggregates. This need is based on the fact that
the particular protein solution becomes cloudy with time and under stressed
conditions and eventually forms a white precipitate. I have not actually
seen this yet, but am basing this on other's observations.
Anyone have any luck with light scatter measured at 340nm?
I am just getting started and could us any suggestions.
Thanks to all, in advance.
James G. Freedy
Senior Analyst
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