PVDF in-situ digestion

From: jaime paba martinez (bucaros@unb.br)
Date: Mon Feb 12 2001 - 14:42:50 EST


Hello,
I am willing to use a tryptic in-situ digestion of some
proteins blotted to PVDF and then to use ES-MS for their
identification. I would appreciate if someone could
suggest a protocol (or a reference paper) to do this.
Thanks in advance for your help.

Jaime Paba
Universidade de Brasilia
Brazil



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