James,
I don't know if this will help or not, but tubulin assembly is commonly
monitored by its turbidity in a spectrophotometer at about 340 nm. One of
the original papers was by Gaskin et al (1974) J. Mol. Biol. 89:737-758.
This is a more organized phenomena than simple aggregation though.
Wilson
At 03:50 PM 2/12/2001 -0500, James_Freedy@hgsi.com wrote:
>Question, anyone have experience with determining aggregate formation in
>protein solutions? I am in need of a good method/instrument to quantitate
>both soluble and insoluble aggregates. This need is based on the fact that
>the particular protein solution becomes cloudy with time and under stressed
>conditions and eventually forms a white precipitate. I have not actually
>seen this yet, but am basing this on other's observations.
>Anyone have any luck with light scatter measured at 340nm?
>I am just getting started and could us any suggestions.
>Thanks to all, in advance.
>
>James G. Freedy
>Senior Analyst
>
>
>
>
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