Stuart,
The other item I did not mention that will be be coming out with the new 3700
productivity pack is a new polymer.
I would bet if you can hold your breath for a couple months the new POP37
polymer will speed things up a bit. The data looked like you could run it much
faster, higher voltage and hotter than the normal POPs with out losing any
resolution.
Whether you will be able to easily migrate the GS runs over to it without
greying your hair is another matter.
hope this helps, Paul
___________________________________
Paul Morrison JFB216............... paul_morrison@dfci.harvard.edu
Molecular Biology Core Facilities..
Dana-Farber Cancer Institute....... http://mbcf.dfci.harvard.edu
44 Binney Street................... 617-632-3082
Boston, MA 02115................... fax 632-4814
-----------------------------------
On Thu, 15 Feb 2001 09:34:32 -0000
"Bayliss, Stuart" <stuart.bayliss@csc.mrc.ac.uk> wrote:
> Morning.
>
> This message sort of tags on to the 3700/3100 core environment use
> discussion that's starting, having purchased a 3700 a year or so ago I'm
> running into a few 'support' difficulties (having said that I'm hoping to
> work something out with the UK ABI guys to be fair...). It's another small
> gripe to add to the list!
>
> I currently run a 3700 in a core facility for both sequencing and genescan
> which means I'm restricted to using POP6 as ABI have never 'officially'
> released POP5 for genescan use.
>
> I'm experiencing a huge demand at the moment and would like to take
> advantage of the quicker POP5 run times, even if it only speeds up
> sequencing and GS runs remain the same....has anyone done such a
> 'validation' exercise? (anyone at ABI care to comment on this or any future
> polymer releases? Sequencing and genescan for us all with POP5 and any new
> releases?). Is there a specific reason not to use POP5 for genescan? From
> what I gather there is just a 'no development' policy for the POP5
> concerning genescan. Please be aware that some users have more than one
> application for the 3700!
>
> Supporting me as a core facility means supporting the whole Institute, and
> greater productivity means more spending on ABI consumables (especially if
> the v3.0 BD's are as good as I'm told!)....I'm quite happy to spend a lot of
> my time doing the validation on our own samples if required.
>
> Thanks for any input.
> Stuart
>
>
> Stuart Bayliss,
> Manager,
> Genetics Core Facility,
> MRC Clinical Science Centre,
> Hammersmith Hospital,
> Du Cane Road,
> London.
> W12 0NN
>
> Tel (lab): 020 8383 3181
> Tel (office): 020 8383 8305
> Fax: 020 8383 8338
> email: stuart.bayliss@csc.mrc.ac.uk
> web: http://gcf.csc.mrc.ac.uk
>
*
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