MS: High Molecular Weight Proteins

From: Kevin Howland (k.howland@ukc.ac.uk)
Date: Mon Feb 19 2001 - 04:43:01 EST


I'm trying to do some MS on a large (>220 kDa) protein that may or may not
exist in multimeric form and am not having much joy/success. I get an
increase in TIC whenever I introduce the sample into the MS but can't seem
to see a discernable mass/charge envelope. (N.B. It needs high salt
conditions to keep it in aqueous solution).

Any suggestions or lit references that may help will be gratefully received.

Thanks in advance

Kevin
 
_______________________________________
Kevin Howland

Protein Science Facility Manager
Research School of Biosciences
University of Kent
Canterbury CT2 7NJ
Tel: +44-1227-764000 ext 7987
Fax: +44-1227-763912
email: k.howland@ukc.ac.uk



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