Don't know about fluorescein, but biotin is a snap. Presumably you're
attaching to the N-terminal, so just treat your biotin as another amino
acid coupling. Works nicely!
Cheers,
Roger
At 03:23 PM 2/19/01 +0000, neil brew (BI) wrote:
>Dear All
> I have got to make some peptides labelled with biotin and
>fluorescein. This will be the first time I have attmpted such labelling and
>I wonder if anybody out there in ABRF-land has any Protocols/references for
>on resin labelling of peptides with such labels as biotin/fluorescein/DIG
>etc.
>
> Many thanks in advance, Neil Brew
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au
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