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From: Maria Lorenzi (mlorenzi@popcsi.unian.it)
Date: Tue Feb 20 2001 - 07:59:54 EST

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I have to ask to you a good protocol to dephosphorylate an yeast plasmid
with an alkaline phosphatase (after digestion of it with one restriction
enzyme EcoRI) before the ligation of it with my fragment of DNA.
Thanks
Maria Lorenzi

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