Neil,
When you put biotin or fluorescein on a peptide, it decreases aqueous
solubility. Both is twice as bad. Consider adding a few hydrophilic AA in
between, also to function as a spacer. I usually make peptides with both
biotin and a dye. The couplings are straightforward.
Mark
-----Original Message-----
From: neil brew (BI) [mailto:neil.brew@bbsrc.ac.uk]
Sent: Monday, February 19, 2001 10:24 AM
To: Recipients of ABRF List
Subject: RE: High Molecular Weight Proteins
Dear All
I have got to make some peptides labelled with biotin and
fluorescein. This will be the first time I have attmpted such labelling and
I wonder if anybody out there in ABRF-land has any Protocols/references for
on resin labelling of peptides with such labels as biotin/fluorescein/DIG
etc.
Many thanks in advance, Neil Brew
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