It works,but if are trying to remove lots of albumin from a rather small
amount of other protein,your chances are not too good.If your protein does
not stick to the resin,you can,at best enrich your prep.
"Helen Kim, Ph.D." <Helen.Kim@ccc.uab.edu>@aecom.yu.edu> on 02/16/2001
04:42:56 PM
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cc:
Subject: Blue-Sepharose
Hi All,
Has anyone had experience removing albumin with Blue-sepharose? I
know in principle it can be done, and I've read lots of papers where
people
blithely say they did with the blue sepharose. I recommended the
procedure to a grad student dealing with blob of albumin in his 2-D gels
of conditioned media. He bought some, tried it (I don't know exactly what
he did), and he says "it didn't work." He's very bright, though, so I
trust
he went through reasonable efforts, but I thought I would check with you
all, since there's no point re-inventing the wheel.
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