Wei Wu,
The best standard at fairly high mass that I am aware of is yeast enolase
(Sigma sells it), but it's only about 46k. If you slightly overload the
sample, you can see the proton-bound dimer at around 92k, though. Accuracy
somewhat depends on what instrument you are running, and more on how clean
your protein is (narrow peaks=better mass accuracy) but I expect <0.1% on
mine for this mass with a good sample. ESI will usually give you better mass
accuracy at this mass, IF you can get the protein to run.
Arnie Falick
Applied Biosystems
FalickAM@appliedbiosystems.com
----- Original Message -----
From: "Wei Wu" <wuwei1@msu.edu>
To: "Recipients of ABRF List" <abrf@aecom.yu.edu>
Sent: Monday, February 19, 2001 7:47 PM
Subject: Big Proteins on MALDI
> Hi Folks,
>
> I'm trying to do MALDI on a pretty big protein (90k). What are the mass
> standards for that range? Are there any matrixes other than sinapinic acid
> that are worth trying for this big protein? Or there are some special
sample
> preparation methods? Is it true that I should not expect a good mass
accuracy
> at this high end?
>
> Thanks in advacne!
>
> Wei Wu
>
> --
> 401 BCH
> MSU
> E. Lansing, MI 48824
> 1-517-353-6767
>
>
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