Dephosphorilaton steps:
(1). Restriction digestion, add CIAP (from Life Technologiy-BRL GIBCO), 1
microliter per 100 microliter reaction mixture, incubate 37C for one hour.
(2). Run a gel to saperate DNA frag. and purify linear DNA from gel with
Agarous Gel Kit from Boehringer or Qiagen
(3). Ligation with your insert....
Yibing
This archive was generated by hypermail 2b29 : Fri Feb 23 2001 - 13:03:34 EST