PepSyn: Y(NO2)

From: Holyst, Trudy (tholyst@bcsew.edu)
Date: Thu Feb 22 2001 - 11:10:32 EST

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Hi All,
I have just synthesized two peptides with Fmoc-Y(3-NO2). The syntheses
seemed to go fine and the HPLC tracings showed one strong peak but the MALDI
TOF mass spec of the purified peak showed the expected mass plus minus 16
and minus 32. Is this an artifact of the mass spec? Is the nitrate group
acid sensitive? I am purifying using RP HPLC at pH 2.

Thanks is advance for your thoughts on this.

Trudy Holyst
Blood Research Institute
Peptide Core Laboratory
Blood Center of SE WI
Milwaukee WI 53201

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