I am using what is basically the same method as yours,except that I use my
reagent synthesized more or less according to the original paper,quite
different gradient and ISCO HPLC.The column is Nova-Pak C18.
I tried different variations on the vapor phase hydrolysis,using the
Waters Pico-Tag hydrolysis apparatus.In all cases,I used 6N HCl with a
variety of additives,since I wanted to get the best recoveries of cysteine
and tryptophan.
I did very little with liquid phase,except for peptides still on resin.Let
me know if you have any preferences,and I could try.
Bohos Mesrob <bmesrob@Tektagen.com>@aecom.yu.edu> on 02/27/2001 09:03:10 AM
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Hello all,
We are using AccQ-Tag derivatization and analysis of protein hydrolyzates
successfully using the Waters Kit and column with HP1100 HPLC System and
gas-phase hydrolysis which is recommended for micro scale AAA.
We would like to check a liquid phase hydrolysis and compare the results
with the gas-phase hydrolysis.
Before beginning the experimental series we would like to have your opinion
and recommendations on these two hydrolysis approaches.
Thanks for your help
Bohos Mesrob, Ph.D.
Senior scientist at
Charles River Tektagen
Phone: 610-640-4550 (ex.123)
Fax: 610-889-9028
e-mailƯ bmesrob@tektagen.com
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