Attn.: recipients of ABRF ListHi Bohos,
You should have a look at the work of Fountoulakis and co-workers concerning protein hydrolysis:
1)Effect of the hydrolysis method on the determination of the amino acid composition of proteins. J. Chromatogr. A 795 (1998) 263-275.
2)Hydrolysis and amino acid composition analysis of proteins (Review). J. Chromatogr. A, 826 (1998), 109-134.
(Gas and liquid)-(conventional and microwave radiation) hydrolysis is compared. Some other intresting topics are discussed as well: Proteins hydrolysis blotted onto PVDF membranes, large-scale hydrolysis etc...
Hope that the above helps,
Kostas
***********************************************************************
Konstantinos PETRITIS
Institut de Chimie Organique et Analytique (ICOA), UMR-6005
University of Orleans, BP 6759, 45067, ORLEANS, CEDEX 2, France
Tel. +(33)-2-38-41-71-71, intern number 7777
Fax: +(33)-2-38-41-72-81
e-mail: konstantinos.petritis@univ-orleans.fr
http://www.univ-orleans.fr/SCIENCES/ICOA
----- Original Message -----
From: Bohos Mesrob
To: Recipients of ABRF List
Sent: Tuesday, February 27, 2001 4:03 PM
Subject: Attn.: recipients of ABRF List
Hello all,
We are using AccQ-Tag derivatization and analysis of protein hydrolyzates successfully using the Waters Kit and column with HP1100 HPLC System and gas-phase hydrolysis which is recommended for micro scale AAA.
We would like to check a liquid phase hydrolysis and compare the results with the gas-phase hydrolysis.
Before beginning the experimental series we would like to have your opinion and recommendations on these two hydrolysis approaches.
Thanks for your help
Bohos Mesrob, Ph.D.
Senior scientist at
Charles River Tektagen
Phone: 610-640-4550 (ex.123)
Fax: 610-889-9028
e-mail bmesrob@tektagen.com
This archive was generated by hypermail 2b29 : Mon Apr 02 2001 - 10:20:49 EDT