Ceramides

From: Paul Jen– (Paul.Jenoe@unibas.ch)
Date: Wed Feb 28 2001 - 08:50:28 EST

Dear Colleagues,

we are trying to measure ceramides in yeasts. After initial fractionation,
we end up with a crude preparation of ceramides in chloroform-methanol 9:1.
When spraying this ceramide preparation we measure abundant singly charged
ions in positive mode with mass to charge ratios of 507, 551, 595, 640,
684, 728, and 772 Da. The mass difference of these ions is 44 Da. Does
somebody have an idea what 44 Da means with respect to ceramide lipids?

Thanks for your input.

Regards

Paul

Paul Jenoe, PhD
Department of Biochemistry
Biozentrum of the University of Basel
Klingelbergstrasse 70
CH-4056 Basel
Switzerland

Tel. +41 61 267 21 57
Fax. +41 61 267 21 48
e-mail Paul.Jenoe@unibas.ch

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