Paul,
In my experience, singly-charged ions spaced at 44 m/z intervals
usually mean that you have surfactant in your sample. However, such ions may
be produced by any polyfunctional or polymeric compound for which the
repeating units form C2H4O, CO2, C3H8 (or even C2HF) upon elimination. If
ceramides fall into this category, then you may be observing your target
compound(s).
Andrew Ross, Ph.D.
Research Officer, Mass Spectrometry
NRC Plant Biotechnology Institute
110 Gymnasium Place
Saskatoon, SK
S7N 0W9 Canada
Tel. (306) 975 6173 (office)
Tel. (306) 975 4193 (lab)
Fax. (306) 975 4839
Email. rossar@pbi.nrc.ca
Adjunct Professor, Biochemistry
University of Saskatchewan
-----Original Message-----
From: Paul Jen– [mailto:Paul.Jenoe@unibas.ch]
Sent: Wednesday, February 28, 2001 7:50 AM
To: Recipients of ABRF List
Cc: Paul Jenoe
Subject: Ceramides
Dear Colleagues,
we are trying to measure ceramides in yeasts. After initial fractionation,
we end up with a crude preparation of ceramides in chloroform-methanol 9:1.
When spraying this ceramide preparation we measure abundant singly charged
ions in positive mode with mass to charge ratios of 507, 551, 595, 640,
684, 728, and 772 Da. The mass difference of these ions is 44 Da. Does
somebody have an idea what 44 Da means with respect to ceramide lipids?
Thanks for your input.
Regards
Paul
Paul Jenoe, PhD
Department of Biochemistry
Biozentrum of the University of Basel
Klingelbergstrasse 70
CH-4056 Basel
Switzerland
Tel. +41 61 267 21 57
Fax. +41 61 267 21 48
e-mail Paul.Jenoe@unibas.ch
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