MetSO

From: Pawel Mak (makp@mol.uj.edu.pl)
Date: Fri Mar 02 2001 - 12:49:08 EST

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Dear colleagues, dear protein chemists,
I need to determine methionine sulphoxide in protein or peptide
hydrolysates on picomole-subnanomole level. Does someone know any effective
protocol based an amino acid analysis techniques and conversion of amino
acids to PTC-derivatives? I hydrolyse the samples in 4 M NaOH, the amino
acids are derivatized with PITC and subjected to separation on Waters
PicoTag column. Unfortunately, the results are very poor. I never can reach
simultaneous determination of MetSO and several other control amino acids.
Any ideas will be greatly appreciated.
Regards,
Pawel

-----------------------------------------------------
Dr Pawel Mak
Jagiellonian University, Institute of Molecular Biology
Mickiewicza 3
31-120 Krakow, POLAND
e-mail: makp@mol.uj.edu.pl
WWW: http://www.mol.uj.edu.pl/biocentrum
fax (+48)(012)6336907

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