Dear Dr. Bystrom
Sypro ruby appears to be one of the most sensitive commercially
available noncovalent fluorescent dyes, but still it's slightly less
sensitive as the best silver protocol and requires very expensive
equipment to read. The main advantage of the ruby dye is the great
reproducibility of the staining process and the linerarity over
approx. 3 orders of magnitude, something you won't get with silver.
Additionally, as ordinary silver protocols are incompatible with post
gel analytics, ruby stain does not preclude trypsin digestion of
proteinspots.
For coomassie and silver use an ordinary desktop scanner (e.g. BioRad
or Pharmacia) for ruby we have found the Fuji laser scanner (approx.
$50'000,-!!) to be the best performer; other models are available by
BioRad and Pharmacia.
One also has to consider, that fluorescent stain is per gel about 10
times more expensive than silver.
Best regards
Dr. Ulrich Schneider
TECAN Proteomics GmbH
Munich / Germany
>Hello,
>
>I'd like to poll the members of this list who are currently imaging
>2D gels for proteomic research. I'd like to know what instrument
>you are using to capture your images with quantitation in mind.
>
>I'm most interested in hearing from those users who are utilizing
>fluorescent stains (esp. Sypro red orange and ruby) but I'm still
>interested in users of silver and coomassie staining.
>
>If you could tell me what instrument you are using and what you feel
>the pros and cons are I would appreciate it and I'll summarize
>responses for the group.
>
>Cheers,
>
>Cory
>
>______________________________
>Dr. Cory Bystrom
>Senior Research Scientist
>New Zealand Dairy Research Institute
>Private Bag 11 029
>Palmerston North
>New Zealand
>
>Voice: 06-350-4600 x7222
>FAX: 06-356-1476
>
>
>
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