native PAGE

From: mdavey (mdavey@tiscalinet.be)
Date: Sat Mar 03 2001 - 14:39:26 EST


Hi there,
I have been asked to separate the products of 2 allelles - peptides
with theoretical pI's of 8.6 and 9, which cannot be resolved by
SDS-PAGE. Does anybody have a reliable protocol for non-denaturing PAGE
of basic proteins please ? - I have had a good look on the interenet but
haven't been able to turn up anything, so all and any advice greatly
recieved,

thanks in advance

Mark

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Mark DAVEY, PhD
e-mail: Mark.Davey@agr.kuleuven.ac.be
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