protseq: insoluble protein

From: Amanda Hall (Amanda.Hall@newcastle.edu.au)
Date: Mon Mar 05 2001 - 00:30:48 EST

Hi everyone,

I have received a sample that is basically a by-product of a casein digestion, I think. It is currently in the form of a solid "filter cake" and is supposed to be insoluble in water. The client wants me to be able to sequence the peptides that are contained within it.

therefore I need suggestions as to ways to make this filter cake soluble so that I can separate the peptides on a HPLC and then collect the peptides for sequencing.Since I want to separate on a C18 column I can't dissolve in any of the conventional solvents.

I have heaps of sample to try out your suggestions

thanks in advance

Amanda

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Amanda Hall-Griffin
Professional Officer
Newcastle Protein
University of Newcastle
Ph (02) 4921-7299
Fax (02) 4921-6903

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