>Hello---
>
>Would anybody share information on their experiences with genotyping
software which is >>compatible with the 3700? Is it necessary to use
Genescan and how well does it work. We >have heard the error rate is close
to 20% (not in our hands) and wondered if other have had >this experience.
Michael,
Our lab has used both ABI377 and ABI 3100 for fluorescent genotyping, and as
far as I know, ABI3700 is identical when it comes to analysis software.
Yes, use of GeneScan is necessary for sizing up fragments. The software
that sorts out fragments is Genotyper. My crew and I spend a lot of time
manually editing peaks that Genotyper identified incorrectly and your number
of 20% incorrect calls seems accurate. The main reason for this is
inconsistent treatment of grouped and ungrouped categories in Genotyper. If
that ever gets fixed, allele calling will be nearly flawless. Please email
me if you would like more information. I will be happy to to share our
experiences.
By the way, PE is coming up with GeneMapper, which is supposed to replace
both GeneScan ad Genotyper. I have a CD of the demo version on my desk, but
for reasons known only to Oracle experts I can't install it - and I tried
four times. Our IT people don't seem to know what is wrong and suspect that
CD may be faulty. Technical support at PE suggested:
1) not enough RAM,
2) wrong service pack for Windows NT (we use 6, they would like to see 5), a
statement that they later retracted, and
3)there is a piece of Oracle somewhere in my computer (they won't say where
to look for it), so I should just get new computer.
Has anybody on this forum installed GeneMapper demo successfully?
Katarzyna Barbara Lobos
RiceTec, Inc.
This archive was generated by hypermail 2b29 : Mon Apr 02 2001 - 10:20:49 EDT