I separated pI 7,8 and about 10 very reproducibly by non-equilibrium
focusing :Meth Enzymol. 169, pp224-233, 1989.
John Holt
Aventis Pasteur
> -----Original Message-----
> From: mdavey [SMTP:mdavey@tiscalinet.be]
> Sent: Saturday, March 03, 2001 2:39 PM
> To: Recipients of ABRF List
> Subject: native PAGE
>
> Hi there,
> I have been asked to separate the products of 2 allelles - peptides
> with theoretical pI's of 8.6 and 9, which cannot be resolved by
> SDS-PAGE. Does anybody have a reliable protocol for non-denaturing PAGE
> of basic proteins please ? - I have had a good look on the interenet but
> haven't been able to turn up anything, so all and any advice greatly
> recieved,
>
> thanks in advance
>
> Mark
>
> ==========================================================================
> ======
>
> Mark DAVEY, PhD
> e-mail: Mark.Davey@agr.kuleuven.ac.be
> ==========================================================================
> ======
>
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