I have recently been trying to detect glycan signals on Finnican LCQDeca
from antibody tryptic glycopeptides. I am using either a "triple play"
experiment or looking at ms/ms of the top 3-5 parent ions from each scan
(both which are tuned for peptide fragmentation). I am relatively new at
looking specifically for the carbohydrate ions an was hoping someone could
give some advice on to the appropriate LCQ settings to optimize for
carbohydrates. I typically cannot find the appropriate mass peak for the
hexoses or sialic acids.
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