Is there any reason why you do base hydrolysis?It is very difficult to deal
with the large volumes produced by neutralization,and some dilution,at
least.Base hydrolysis tends to destroy a number of amino acids,so that is
not good either.Barium hydroxide is better than sodium hydroxide,because it
can neutralized with sulfuric acid,giving an insoluble barium sulfate.It
has been my experience that some amino acids are trapped in the
precipitate.So,I am back to the original question.
Pawel Mak <makp@mol.uj.edu.pl>@aecom.yu.edu> on 03/02/2001 06:49:08 AM
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Subject: MetSO
Dear colleagues, dear protein chemists,
I need to determine methionine sulphoxide in protein or peptide
hydrolysates on picomole-subnanomole level. Does someone know any effective
protocol based an amino acid analysis techniques and conversion of amino
acids to PTC-derivatives? I hydrolyse the samples in 4 M NaOH, the amino
acids are derivatized with PITC and subjected to separation on Waters
PicoTag column. Unfortunately, the results are very poor. I never can reach
simultaneous determination of MetSO and several other control amino acids.
Any ideas will be greatly appreciated.
Regards,
Pawel
-----------------------------------------------------
Dr Pawel Mak
Jagiellonian University, Institute of Molecular Biology
Mickiewicza 3
31-120 Krakow, POLAND
e-mail: makp@mol.uj.edu.pl
WWW: http://www.mol.uj.edu.pl/biocentrum
fax (+48)(012)6336907
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