Mark
Try the classical acetic acid /urea gels used very succesfully for histones
and
their polymorphic variants
Panyim and Chalkley J Biol Chem. 1971, 246(13)4206-15.
There was a modification by W. Bonner late '70s or early '80s(unfortunately
don't
have the reference)- some amount of TX-100 in the gel allowed separation of
a
single AA polymorphism
Lyuben
Lyuben MarekovÝ
NIAMS, Bldg6, Room 128
Phone 301-496-7190
e-mail: marekovl@mail.nih.gov
> -----Original Message-----
> From: John.Holt@aventis.com [mailto:John.Holt@aventis.com]
> Sent: Monday, March 05, 2001 10:49 AM
> To: Recipients of ABRF List
> Subject: RE: native PAGE
>
>
> I separated pI 7,8 and about 10 very reproducibly by non-equilibrium
> focusing :Meth Enzymol. 169, pp224-233, 1989.
>
> John Holt
>
> Aventis Pasteur
>
> > -----Original Message-----
> > From: mdavey [SMTP:mdavey@tiscalinet.be]
> > Sent: Saturday, March 03, 2001 2:39 PM
> > To: Recipients of ABRF List
> > Subject: native PAGE
> >
> > Hi there,
> > I have been asked to separate the products of 2 allelles -
> peptides
> > with theoretical pI's of 8.6 and 9, which cannot be resolved by
> > SDS-PAGE. Does anybody have a reliable protocol for
> non-denaturing PAGE
> > of basic proteins please ? - I have had a good look on the
> interenet but
> > haven't been able to turn up anything, so all and any advice greatly
> > recieved,
> >
> > thanks in advance
> >
> > Mark
> >
> >
> ==============================================================
> ============
> > ======
> >
> > Mark DAVEY, PhD
> > e-mail: Mark.Davey@agr.kuleuven.ac.be
> >
> ==============================================================
> ============
> > ======
> >
>
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