Hi Pawel,
An effective method for determining MetSO in proteins is given in:
Levine, RL et al., Proc.Natl.Acad.Sci. USA, 93:15036-15040 (1996)
It uses CNBr treatment of the protein. This destroys Met but not
MetSO. The protein is then hydrolyzed with 6 M HCl, which converts MetSO to
Met. The Met is determined by amino acid analysis after removing the HCl.
I have used this method and it works very well.
Daniel
At 12:49 PM -0500 3/2/01, Pawel Mak wrote:
>Dear colleagues, dear protein chemists,
>I need to determine methionine sulphoxide in protein or peptide
>hydrolysates on picomole-subnanomole level. Does someone know any effective
>protocol based an amino acid analysis techniques and conversion of amino
>acids to PTC-derivatives? I hydrolyse the samples in 4 M NaOH, the amino
>acids are derivatized with PITC and subjected to separation on Waters
>PicoTag column. Unfortunately, the results are very poor. I never can reach
>simultaneous determination of MetSO and several other control amino acids.
>Any ideas will be greatly appreciated.
>Regards,
>Pawel
>
>-----------------------------------------------------
>Dr Pawel Mak
>Jagiellonian University, Institute of Molecular Biology
>Mickiewicza 3
>31-120 Krakow, POLAND
>e-mail: makp@mol.uj.edu.pl
>WWW: http://www.mol.uj.edu.pl/biocentrum
>fax (+48)(012)6336907
Daniel Wellner, Ph.D.
Department of Biochemistry, Room A-284
Weill Medical College of Cornell University
New York, NY 10021
Tel: (212) 746-6409
FAX: (212) 746-8875
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