Dear Synthesisers
I have been give the task of inducing succinimide formation in a peptide
instead of preventing it . The site is .....Asp-Asn-Ala-OH at the
C-terminus of a 8AA peptide. Will extended piperidine treatment be
sufficient to generate 100% succinimide or should I use DBU. I guess I
won't have a problem partially forming succinimide but I want to drive the
reaction as close to 100% as I can. I am also aware that on the aqueous
workup that i must avoid hydrolysing the product. I know that low pH makes
it stable but how stable? Is there anyone out there who has faced a similar
synthetic dilemma?
Denis Scanlon
Auspep Pty Ltd
Auspep Pty Ltd
115 Ireland St
West Melbourne
Australia 3003
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