Are you asking why you are seeing anything at all in the protease inhibitor
tablets? If so, wouldn't it be because there are peptides in there?
Helen Kim, Ph.D.
Res. Associate Professor
Department of Pharmacology & Toxicology
Director, 2-D Proteomics Laboratory
University of Alabama at Birmingham
1670 University Blvd, Volker Hall, G78P
Birmingham, AL 35294-0019
(205) 934-3880 (office, with voicemail)/5-3244 (lab, no voicemail)
FAX: (205) 934-6944
helenkim@uab.edu
> ----------
> From: Jonathan Xu
> Sent: Wednesday, March 7, 2001 11:03 AM
> To: Recipients of ABRF List
> Subject: cell lyses protocol for MALDI MS
>
> Dear ABRF members,
> I am working on a mouse colon crypts cell lyses protocol for MALDI
> analysis.
> I am using the complete protease inhibitor cocktail tablets. The MALDI
> spectrum only shows about 100 protein. Also the protease inhibitor have a
> polymeric pattern from 5000-7500 m/z with a 45 da difference each peak. If
>
> you have any advice on how to get the cell lyses protocol for MALDI, or
> why
> the complete protease inhibitor tablet have a polymeric pattern, I would
> be
> very appreciate your help.
>
> Regards,
> Jonathan Xu
>
>
> Mass Spectrometry Research Center
> Vanderbilt University Medical Center
> Nashville, TN 37232
> Tel (615)343-8430
> Fax (615)343-8372
>
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