Hi Katarzyna:
I installed a demo version of Genemapper on a new windows NT system in our
lab. The install went fine. So far, I have not had a lot of time to play
with the software. Problems I did notice: my project kept being renamed
"green" and none of the information within the sample files' "sample
comments" and "sample info" fields showed up in Genemapper. The software
also crashed once.
I am waiting for a response from my field applications specialist.
Lynn
>>Hello---
>>
>>Would anybody share information on their experiences with genotyping
>software which is >>compatible with the 3700? Is it necessary to use
>Genescan and how well does it work. We >have heard the error rate is close
>to 20% (not in our hands) and wondered if other have had >this experience.
>
>
>Michael,
>
>Our lab has used both ABI377 and ABI 3100 for fluorescent genotyping, and as
>far as I know, ABI3700 is identical when it comes to analysis software.
>Yes, use of GeneScan is necessary for sizing up fragments. The software
>that sorts out fragments is Genotyper. My crew and I spend a lot of time
>manually editing peaks that Genotyper identified incorrectly and your number
>of 20% incorrect calls seems accurate. The main reason for this is
>inconsistent treatment of grouped and ungrouped categories in Genotyper. If
>that ever gets fixed, allele calling will be nearly flawless. Please email
>me if you would like more information. I will be happy to to share our
>experiences.
>
>By the way, PE is coming up with GeneMapper, which is supposed to replace
>both GeneScan ad Genotyper. I have a CD of the demo version on my desk, but
>for reasons known only to Oracle experts I can't install it - and I tried
>four times. Our IT people don't seem to know what is wrong and suspect that
>CD may be faulty. Technical support at PE suggested:
>1) not enough RAM,
>2) wrong service pack for Windows NT (we use 6, they would like to see 5), a
>statement that they later retracted, and
>3)there is a piece of Oracle somewhere in my computer (they won't say where
>to look for it), so I should just get new computer.
>
>Has anybody on this forum installed GeneMapper demo successfully?
>
>Katarzyna Barbara Lobos
>RiceTec, Inc.
>
Lynn Petukhova
Assistant Director
Genotyping Core Facility
Starr Center for Human Genetics
The Rockefeller University
1230 York Avenue, Box 241
New York, NY 10021
phone: (212) 327-7181
fax: (212) 327-7182
This archive was generated by hypermail 2b29 : Mon Apr 02 2001 - 10:20:49 EDT