maximum SDS concentration for hydrolysis and ninbydrin detection( CIE)?

From: Buschman, Frank (Frank_Buschman@chiron.com)
Date: Mon Mar 12 2001 - 20:23:27 EST

What are the considerations to take into accout when hydrolyzing protein in
the presents of SDS. I am intrested in detection using a Beckman 6300 amino
acid analyzer. What is the maximum amount(or concentration) of SDS that the
hydrolysis can tolerate. I presume that liquid phase hydrolysis tolerates
mores SDS than does vapor phase hydrolysis, is this true? Does anyone
understand the mechanism of SDS interference with hydrolysis? flb

This archive was generated by hypermail 2b29 : Mon Apr 02 2001 - 10:20:49 EDT