Hi everybody,
I've just completed a tryptic digest where the addition of the trypsin
turned the digestion solution dark blue, I've been told that this sort of
reaction can happen where there are particularly high concentrations of
certain amino acids - apparently in this case tryptophan. I'm intrigued,
can anyone expand on what exactly is going on for me?
The gel piece was initially stained with coomassie, I destained it with 40%
ethanol in 50mM amm. bicarb. and then washed it with 25mM amm. bicarb.,
dehydrated with acetonitrile and then added the trypsin in 25mM amm. bicarb.
Thanks,
Derek Bradley
Dept. of Medicine
UCL
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