Hi Jane
We have used the Centricon Plus-80s on a regular basis over the
past 2 years for concentrating folded protein complexes after dialysis. We
have never tried to autoclave them, but we have never had a problem with
sterility. We do keep our dialysate on ice at all times and loaded with
protease inhibitors.
Scottie
At 11:48 AM -0800 3/13/01, Jane Moon wrote:
>I'm new to protein purification and am following old protocols from
>elsewhere. I've been using Centricon Plus-80s for concentration but am
>concerned about sterility. Millipore says that they don't plan on
>sterilizing but give an unvalidated protocol for autoclaving. Doesn't
>anyone else worry about sterility? If not, why not? And I've heard mixed
>reviews about this product. Your opinion?
>
>Thanks,
>-Jane.
Scottie Adams aka Pamela Scott Adams
Manager
Molecular Biology Core Facility
Trudeau Institute
100 Algonquin Avenue
Saranac Lake, NY 12983
Phone: 518-891-3080, Ext. 115
Fax: 518-891-5126
Email: sadams@northnet.org
sadams@trudeauinstitute.org
http://www.trudeauinstitute.org
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