"William H. Vensel" wrote:
>
> Hello,
>
> We are considering purchasing an HPLC system capable of operating in the
> nanoliter to microliter flow range. Our hope is to be able to use it with
> our LCQ Classic. We want to be able to use a combination of stepwise ion
> exchange chromatography followed by reverse phase HPLC to characterize
> peptides from tryptic digests. There are two instruments that we are
> looking at, one from LC-packings and the other from Michrom. The
> instruments are similar in that they both have detectors, autosamplers and
> switching valves. Would people that have had experience with these
> instruments or others please let me hear from you regarding thir
> performance and reliability. I will post the responses after removing
> identifying information.
>
> Thank you
> Bill
> USDA/WRRC
> Albany, CA
Have you considered Microtech Scientific or Waters? Both have capillary
HPLC systems. We have four systems here: a Waters 600MS that we split
for capillary columns and infuse into our LCQ Classic; a Microtech that
was connected to an LCQ Deca, a Waters CapLC that is being connected to
the LCQ Deca; and a Micromass CapLC connected to our Q-TOF.
We are in the process of connecting the Waters CapLC to the Deca for
increased throughput. The Waters CapLC has an autosampler with three
injection modes of which the uL pickup mode is of interest to us. If we
get it running consistently it will be nice but we have had a series of
problems with the pumps and autosampler.
The Microtech has served us well. We ran it at a higher flow rate (10
uL/min) and split it for use with capillary columns.
The Micromass CapLC is basically the same instrument as the Waters CapLC
(go figure) but is better suited for connection to the Q-TOF (another no
brainer). So far the Micromass system is running much better that the
Waters system but we have not used it as much. Time will tell on that
one.
Hope this helps.
Damon
-- Damon Barbacci, Ph.D. Washington University Mass Spectrometry Facility Department of Chemistry, Campus Box 1134 One Brookings Drive St. Louis, MO 63130
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