> At 09:06 AM 3/14/01, Bob Lyons wrote:
> >Thomas J Stelick wrote:
> > >
> > > hello,
> > > We have been getting some inconsistant results on some of our sequencing
> > > and one problem may be the lot of big dye. The problem showed up around
> > > the time we started using a new lot. The lot # is 0101047. Is anyone else
> > > having problems. We are using a 3700. Or is anyone having a problem with
> > > pop-6 polymer lot # 0101130.
> >
> >
> >We have been questioning POP6 lot 0101130 ourselves. An instrument that was
> >working very well with read lengths of 700-800 suddenly changed to reads of
> >350-450 after only a POP6 lot switch. ABI had no other reports of a problem
> >with that lot.....
Having shot off my mouth about a possible bad POP lot, I want to make sure
I also present the opposite side. Yesterday, we regenerated our array, and
the next set of plates we ran - BD standards followed by 300 client samples -
gave really nice reads. This is still with that POP6 lot 0101130 on which
I was casting aspersions mere hours ago. I'm still baffled as to how a POP
lot change could have caused our previous problems (basically toasted the
array) but it is clear that this lot of POP *can* produce some nice results.
Anyone else with a similar experience regarding serious loss of resolution
following a POP lot change?
Bob Lyons
University of Michigan
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Robert Lyons, Ph.D.
Director, DNA Sequencing Core
University of Michigan
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This archive was generated by hypermail 2b29 : Mon Apr 02 2001 - 10:20:49 EDT