see http://www.abrf.org/archives/hmail/97006/0028.html
also rong-rong zhu at basf posted questions on this
issue as well
http://www.abrf.org/archives/hmail/0021/0213.html
for contact info
you might look at
http://www.chemistry.gatech.edu/stms/bigprot.txt
also ...
issues: the charge envelope is likely 2500-4500, the thing is trapping salts and buffers
like crazy, there is micro-heterogeneity spreading the signal, you need a plrp and a trap
cartridge, you need to sum/avg alot of scans from the chromatogram off the plrp, you need
WAY more material, INFUSION will NEVER NEVER WORK...desalted or not REALLY! LC/MS with a
trap and PLRP is the only way you will see a nice signal...hard sample.
Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software
-----Original Message-----
From: Scott Borneman <sborneman@epicyte.com>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Friday, March 16, 2001 4:17 PM
Subject: MS Deconvolution of Large Proteins
>I have had some success in deconvoluting proteins in the mass range of 15-30
>KDa on a LCQ Deca by infusion of desalted proteins in high concentration of
>acetic acid and CH3CN. Lately I have been attempting to run large 150,000
>da proteins using a variety of ESI settings and different solvents without
>any success. If anyone out there has had better luck or could recommend
>some setting to improve ESI of large proteins on LCQ Deca I would love to
>hear about it.
>
>Thanks in advance
>
>
>
>
>
>Scott Borneman, Ph.D.
>
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